The goal of most gene therapies is to insert a healthy copy of a gene into the genome. Besides CRISPR, techniques for integrating DNA into the genome do not target that DNA to a particular genomic location, and thus different cells end up with the DNA sequence in different positions.
A. It has been shown that where the healthy gene integrates is heavily influenced by chromatin. Why?
B. The same gene integrated into different locations in the genome will be expressed differently. Why?
C. Over time the expression of an integrated gene will change, usually decreasing. Describe how you think the cell downregulates expression of an integrated gene.
The genome of E. Coli is approximately 5 x 10 bp. How many bands would you expect to see in an EcoR I or Hind III digest of E. coli DNA?
Judge the usefulness of direct and indirect methods to measure microbial growth.
